The Research Core Facility at LSU Health Shreveport provides investigators, post-doctoral fellows, and graduate and undergraduate students with access to a wide variety of research services, educational/training opportunities and instrumentation.

Located on the 6th floor of the Biomedical Research Institute, each technology is staffed by trained research specialists.  These technologies include flow cytometry, laser capture microdissection, mass spectrometry, microarray, fluorescence, confocal, and super resolution confocal microscopy, next generation sequencing, and real-time PCR. The Research Core Facility supports both the experienced and novice investigator.

We encourage you to explore our facility as well as other Core Facilities on our campus.

Microscopy

Leica TCS SP5 Spectral Confocal Microscope

Nikon A1R Confocal & N-SIM Super Resolution System

Nikon Eclipse TE300 Widefield Microscope

Zeiss AxioObserver Z1 Widefield/Apotome Microscope
 
Zeiss LSM 510 NLO Confocal/Multiphoton Microscope

Learn more

Flow Cytometry

BD Biosciences FACSAriaIII Flow Cytometer/Cell Sorter

BD Biosciences FACSCalibur Flow Cytometer

BD Biosciences LSRII Flow Cytometer

BD Biosciences LSRII-SORP Flow Cytometer

Learn more

Genomics

Microarray
Affymetrix GeneChip System

Laser Capture Microdissection
Arcturus XT LCM System 

Next Generation Sequencing
Illumina MiSeq Sequencer
Illumina NextSeq 550 Sequencer

Real-Time Quantitative PCR
Bio-Rad CFX96 Fast Real-Time PCR Instruments (3)
Bio-Rad CFX384 Touch Real-Time PCR Instrument

Learn more

Mass Spectrometry

Thermo Finnigan Deca XP Max Nanospray LC/MS Mass Spectrometer

Waters SYNAPT High-Resolution Mass Spectrometer

Waters Q-TOF Micro Mass Spectrometer

Learn more

 

Equipment Description & Usage

REAL-TIME QUANTITATIVE PCR

Scientific Advisor: Dr. Rona Scott
Genomics Research Specialist: Camille Abshire, MS, MDXT-(AAB), CLS (LSBME)

Bio-Rad CFX96
The RCF currently houses three Bio-Rad CFX96 instruments. The CFX96 is a six-channel real-time PCR system that combines advanced optical technology with precise thermal control to deliver sensitive, reliable detection. The system’s solid-state optical technology (six filtered LEDs, each with a corresponding filtered photodiode) maximizes fluorescence detection for specific dyes in specific channels, providing sensitive detection for quantification and target discrimination. Data are collected from all wells during data acquisition. At every position and with every scan, the optics shuttle is reproducibly centered above each well, so the light path is always optimal and there is no need to sacrifice data collection on one of the channels to normalize to a passive reference. Users can select multiple data acquisition modes, including a one-color fast scan for SYBR green. Thermal gradient features can be used to optimize reactions in a single run. The new CFX Manager software has advanced analysis tools for performing normalized gene expression. In addition, this system does not require fluorescein or ROX for instrument normalization.

Bio-Rad CFX384
The Research Core Facility has one Bio-Rad CFX384 Touch Real-Time PCR System.  The system's solid-state optical technology (5 filtered LEDs and 5 filtered photodiodes) provide precise quantification and multiplex target discrimination.  All other acquisition and analysis parameters are identical to the CFX96 Fast System.

CONTACT US

Kelly Tatchell, PhD
Director, Research Core Facility
Associate Dean of Graduate Studies
ktatch@lsuhsc.edu
(318) 675-7769

Paula Polk, MS
Assistant Director, Research Core Facility
ppolk@lsuhsc.edu
(318) 675-4939

Camille Abshire, MS, MDxT-(AAB), CLS (LSBME)
Research Specialist, Genomics
cabsh2@lsuhsc.edu
(318) 675-4174

David Custis, MS
Research Specialist, Flow Cytometry
dcusti@lsuhsc.edu
(318) 675-4174

Chaowei Shang, PhD
Research Specialist, Microscopy
cshang@lsuhsc.edu
(318) 675-8537

Xinggui Shen, PhD
Mass Spectroscopy Specialist
Assistant Professor, Pathology
xshen@lsuhsc.edu
(318) 675-3371

Ana-Maria Dragoi, MD, PhD
Associate Director, INLET
Feist-Weiller Cancer Center 
adrag1@lsuhsc.edu
(318) 675-4216

Jennifer Carroll, PhD
Translational Research Specialist, INLET
Feist-Weiller Cancer Center 
jcarro1@lsuhsc.edu
(318) 675-4234

Research Core Facility

Research Core Facility

Research Core Facility

Research Core Facility