The Research Core Facility at LSU Health Shreveport provides investigators, post-doctoral fellows, and graduate and undergraduate students with access to a wide variety of research services, educational/training opportunities and instrumentation.

Located on the 6th floor of the Biomedical Research Institute, each technology is staffed by trained research specialists.  These technologies include flow cytometry, laser capture microdissection, mass spectrometry, microarray, fluorescence, confocal, and super resolution confocal microscopy, next generation sequencing, and real-time PCR. The Research Core Facility supports both the experienced and novice investigator.

We encourage you to explore our facility as well as other Core Facilities on our campus.

Microscopy

Leica TCS SP5 Spectral Confocal Microscope

Nikon A1R Confocal & N-SIM Super Resolution System

Nikon Eclipse TE300 Widefield Microscope

Zeiss AxioObserver Z1 Widefield/Apotome Microscope
 
Zeiss LSM 510 NLO Confocal/Multiphoton Microscope

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Flow Cytometry

BD Biosciences FACSAriaIII Flow Cytometer/Cell Sorter

BD Biosciences FACSCalibur Flow Cytometer

BD Biosciences LSRII Flow Cytometer

BD Biosciences LSRII-SORP Flow Cytometer

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Genomics

Microarray
Affymetrix GeneChip System

Laser Capture Microdissection
Arcturus XT LCM System 

Next Generation Sequencing
Illumina MiSeq Sequencer
Illumina NextSeq 550 Sequencer

Real-Time Quantitative PCR
Bio-Rad CFX96 Fast Real-Time PCR Instruments (3)
Bio-Rad CFX384 Touch Real-Time PCR Instrument

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Mass Spectrometry

Thermo Finnigan Deca XP Max Nanospray LC/MS Mass Spectrometer

Waters SYNAPT High-Resolution Mass Spectrometer

Waters Q-TOF Micro Mass Spectrometer

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Equipment Description & Usage

NEXT-GENERATION SEQUENCING

Scientific Advisor: Dr. Rona Scott
Genomics Research Specialist: Camille Abshire, MS, MDXT-(AAB), CLS (LSBME)

Illumina MiSeq
The Illumina MiSeq is a next-generation DNA sequencer with a single-lane flow cell. It is capable of generating up to 25 million reads with up to 15GB of output in a single run. It can produce 2 x 300 paired end reads. It has the output to accommodate targeted gene sequencing, metagenomics, small genome sequencing, targeted gene expression and amplicon sequencing. Sample libraries are loaded directly onto the MiSeq where amplified clusters are generated on the flow cell, followed by sequencing by synthesis. Samples can be barcoded and multiplexed or pooled together in the same lane.

Illumina NextSeq 550
The Illumina NextSeq 550 is a high-throughput sequencer that uses sequencing by synthesis technology, a highly accurate reversible-terminator technology.  This technology uses fluorescently labeled nucleotides to sequence hundreds of millions of clusters on a flow cell surface in parallel.  This method greatly reduces the number of errors and missed calls associated with homopolymers.  The NextSeq 550 integrates cluster generation and sequencing into a single instrument, generating base calls and quality metrics in real time.  Data are automatically transferred to the BaseSpace environment for simplified analysis, storage, and sharing.  The NextSeq 550 is flexible, offering tunable read lengths and output options to support individual research needs.  This system has a maximum output of up 120GB with up to 400 million sequencing reads.  Applications include genomic DNA sequencing, exome sequencing, mRNA sequencing, small RNA/microRNA sequencing, epigenetics, small genome sequencing and targeted resequencing.

CONTACT US

Kelly Tatchell, PhD
Director, Research Core Facility
Associate Dean of Graduate Studies
ktatch@lsuhsc.edu
(318) 675-7769

Paula Polk, MS
Assistant Director, Research Core Facility
ppolk@lsuhsc.edu
(318) 675-4939

Camille Abshire, MS, MDxT-(AAB), CLS (LSBME)
Research Specialist, Genomics
cabsh2@lsuhsc.edu
(318) 675-4174

David Custis, MS
Research Specialist, Flow Cytometry
dcusti@lsuhsc.edu
(318) 675-4174

Chaowei Shang, PhD
Research Specialist, Microscopy
cshang@lsuhsc.edu
(318) 675-8537

Xinggui Shen, PhD
Mass Spectroscopy Specialist
Assistant Professor, Pathology
xshen@lsuhsc.edu
(318) 675-3371

Ana-Maria Dragoi, MD, PhD
Associate Director, INLET
Feist-Weiller Cancer Center 
adrag1@lsuhsc.edu
(318) 675-4216

Jennifer Carroll, PhD
Translational Research Specialist, INLET
Feist-Weiller Cancer Center 
jcarro1@lsuhsc.edu
(318) 675-4234

Research Core Facility

Research Core Facility

Research Core Facility

Research Core Facility