The Research Core Facility at LSU Health Shreveport provides investigators, post-doctoral fellows, and graduate and undergraduate students with access to a wide variety of research services, educational/training opportunities and instrumentation.
Located on the 6th floor of the Biomedical Research Institute, each technology is staffed by trained research specialists. These technologies include flow cytometry, laser capture microdissection, mass spectrometry, microarray, fluorescence, confocal, and super resolution confocal microscopy, next generation sequencing, and real-time PCR. The Research Core Facility supports both the experienced and novice investigator.
We encourage you to explore our facility as well as other Core Facilities on our campus.
Leica TCS SP5 Spectral Confocal Microscope
Nikon A1R Confocal & N-SIM Super Resolution System
Nikon Eclipse TE300 Widefield Microscope
Zeiss AxioObserver Z1 Widefield/Apotome Microscope
Zeiss LSM 510 NLO Confocal/Multiphoton Microscope
BD Biosciences FACSAriaIII Flow Cytometer/Cell Sorter
BD Biosciences FACSCalibur Flow Cytometer
BD Biosciences LSRII Flow Cytometer
BD Biosciences LSRII-SORP Flow Cytometer
Affymetrix GeneChip System
Laser Capture Microdissection
Arcturus XT LCM System
Next Generation Sequencing
Illumina MiSeq Sequencer
Illumina NextSeq 550 Sequencer
Real-Time Quantitative PCR
Bio-Rad CFX96 Fast Real-Time PCR Instruments (3)
Bio-Rad CFX384 Touch Real-Time PCR Instrument
Thermo Finnigan Deca XP Max Nanospray LC/MS Mass Spectrometer
Waters SYNAPT High-Resolution Mass Spectrometer
Waters Q-TOF Micro Mass Spectrometer
Equipment Description & Usage
Scientific Advisor: Dr. Kevin Lin
Microscopy Research Specialist: Dr. Chaowei Shang
Nikon A1R Confocal & SIM Super Resolution System
This is a high-speed Nikon A1R confocal microscope combined with a SIM (Structured Illumination Microscopy) super resolution system for fixed and live sample-imaging. The Nikon Eclipse Ti-E inverted microscope is built with fully automated objectives, stage, and filter turrets. The 5 objectives range from 10X to 100X. The confocal components include 5 detectors and 4 lasers: 405nm, 488nm, 561nm, and 640nm in wavelength. The SIM components include a Hamamatsu camera and 3 LED lasers: 488nm, 561nm, and 640nm in wavelength. DAPI images can still be taken under SIM mode. The microscope is capable for bright-field imaging, multichannel fluorescence imaging, large image stitching, multipoint acquisition, time-lapse acquisition, FRAP, FRET imaging, and 2D/3D deconvolution. The resolution for conventional confocal reaches 200-250nm, while the resolution for super resolution system reaches 85-100nm. To allow long hour live cell-imaging, the microscope is housed in an incubator with CO2, O2, and humidity controls.
Leica TCS SP5 Confocal Microscope
This is a very flexible and fast confocal system for fixed or living samples. The system includes a Leica DMI 6000 CS fully automated inverted microscope with motorized stage, condenser, objective, and filter turrets. The microscope is housed in a Ludin full enclosure incubator with an internal Ludin Cube2 with CO2, O2, and humidity controls. It is equipped with 5 lasers for excitation: violet diode (405 nm), multi-line Argon (458, 476, 488, 496, and 514 nm), green HeNe (543 nm), orange HeNe (594 nm), and red HeNe (633 nm); visualization is done using a mercury arc lamp. The spectral beam splitter has freely adjustable bandwidths for the collection of signal in 5 separate detectors simultaneously or sequentially. There is also a transmitted light detector for DIC. There are 9 available objectives, ranging from a 2.5x to a Plan Apo 100x/1.46 NA oil objective. The system runs on the newest version of LAS AF software (LAS AF 2.6.3), with FRAP, FRET, Mark & Find, 3D Visualization, Colocalization, and Live Data Modes.
Zeiss AxioObserver/Apotome Microscope
The system is built around a Zeiss AxioObserver Z1 inverted fluorescent microscope, fully automated, with component recognition to minimize errors. System components include mercury arc lamp excitation, a Zeiss AxioCamMRm CCD camera with 12-bit dynamic range, extended sensitivity in the near infrared, a fully automated XYZ stage, a complement of objectives ranging from 10x to 100x, and five installed filter sets for DAPI, FITC, narrow band GFP, Rhodamine, and Far Red. The Apotome attachment is designed for precise optical sectioning. The Apotome slides easily into the optical path and projects a grid onto the image plane, which is shifted laterally in three defined steps, with an image collected at each step. A software algorithm then removes any out-of-focus signal. The acquisition software is AxioVision v.4.8, including plug-in options for Inside 4D, 3D Deconvolution, Colocalization, Mark & Find, Mosaic, and more.
Nikon Widefield Microscope
The system is built around a Nikon Eclipse TE300 inverted microscope with a range of objectives for phase, DIC, and high resolution epifluorescent imaging. The software package controls a Prior OptiscanTM XYZ stage with a full complement of stage inserts and a Prior filter wheel containing excitation filters from the Chroma 83000 filter set. This set includes single and multiband excitation filters for DAPI, FITC, GFP, Texas Red, Rhodamine, or PI. Fluorescent images are acquired with a black and white ANDOR Neo/Zyla camera; high resolution color images may also be acquired with the DS Fi2 color camera. The stage, filters, shutters, and camera are controlled by the NIS Elements software from Nikon.
Zeiss LSM 510 NLO Confocal/Multiphoton Microscope
The Zeiss LSM 510 NLO system is configured to enhance living tissue research. The scanning system is connected to an upright Axioskop 2 FS MOT microscope equipped with a set of objectives selected for physiological measurements and live animal studies. The stage remains in a fixed position, and the objectives have motorized focus control. It is equipped with the following lasers and laser lines for excitation: Argon (458, 477, 488, 514 nm), HeNe (543 nm), HeNe (633 nm), and the Coherent Chameleon-XR Ti: Sapphire laser (tunable from 705 to 980 nm). The ultrafast-pulsed Chameleon laser emitting NIR radiation allows imaging up to 500 μm deep within tissue. There are three PMTs for visible wavelength detection, a transmitted light detector, and two non-descanned detectors for multiphoton imaging. The LSM 510 ZEN operating software includes the Physiology v3.5 and Image Visart v3.5 options that permit 2D, 3D, and 4D image collection and processing, 3D/4D animation, calibration and measurement of ion concentrations, time series analysis, and graphical mean-of-ROI analysis.
Off-line Image Analysis Stations There are two computer stations in the Core Facility Computer Lab reserved for microscope users, which are loaded with specialized imaging software. The RCFoffline2 system (Fujitsu/Siemens Celsius R650) has two processors, four terabyte hard drives, and an ATI Fire GL V7350 video card with 1 GB of on-board memory. It runs on Windows XP Professional (32 bit) and is loaded with Media Cybernetics’ AutoQuant AutoDeblur deconvolution software (AutoQuant X3), including the AutoVisualize option, and full off-line versions of Zeiss LSM 510 AIM (confocal) and Zeiss Axiovision 4.8.2 (AxioObserver with Apotome) software. RCFoffline1, a Dell Optiplex 3010, runs on Windows 7 Professional (64 bit) and is loaded with Leica LAS AF 2.6.3, NIS Elements AR 4.13.04, and Zeiss LSM 510 ZEN 2009.