The Research Core Facility at LSU Health Shreveport provides investigators, post-doctoral fellows, and graduate and undergraduate students with access to a wide variety of research services, educational/training opportunities and instrumentation.
Located on the 6th floor of the Biomedical Research Institute, each technology is staffed by trained research specialists. These technologies include flow cytometry, laser capture microdissection, mass spectrometry, microarray, fluorescence, confocal, and super resolution confocal microscopy, next generation sequencing, and real-time PCR. The Research Core Facility supports both the experienced and novice investigator.
We encourage you to explore our facility as well as other Core Facilities on our campus.
Leica TCS SP5 Spectral Confocal Microscope
Nikon A1R Confocal & N-SIM Super Resolution System
Nikon Eclipse TE300 Widefield Microscope
Zeiss AxioObserver Z1 Widefield/Apotome Microscope
Zeiss LSM 510 NLO Confocal/Multiphoton Microscope
BD Biosciences FACSAriaIII Flow Cytometer/Cell Sorter
BD Biosciences FACSCalibur Flow Cytometer
BD Biosciences LSRII Flow Cytometer
BD Biosciences LSRII-SORP Flow Cytometer
Affymetrix GeneChip System
Laser Capture Microdissection
Arcturus XT LCM System
Next Generation Sequencing
Illumina MiSeq Sequencer
Illumina NextSeq 550 Sequencer
Real-Time Quantitative PCR
Bio-Rad CFX96 Fast Real-Time PCR Instruments (3)
Bio-Rad CFX384 Touch Real-Time PCR Instrument
Thermo Finnigan Deca XP Max Nanospray LC/MS Mass Spectrometer
Waters SYNAPT High-Resolution Mass Spectrometer
Waters Q-TOF Micro Mass Spectrometer
Equipment Description & Usage
Scientific Advisor: Dr. Rona Scott
Genomics Research Specialist: Camille Abshire, MS, MDXT-(AAB), CLS (LSBME)
Affymetrix GeneChip System
This system is suitable for global gene expression studies using the Affymetrix GeneChip Probe arrays. Oligonucleotide arrays, prepared on glass, are hybridized to biotinylated probes prepared from biological samples and detected with a fluorescent label. Probes for these experiments are derived from a single source, and differentially expressed genes are identified by comparing the results of experiments performed with different chips. A major advantage of this approach is the ready availability of pre-prepared arrays representing a large number of sequences from a number of species.
This system consists of the following components:
1. A GeneChip Hybridization Oven 640 for automated control of hybridization to the GeneChip arrays.
2. A GeneChip Fluidics Station 450 for automated washing of chips and labeling of hybridized probes. This station can wash and stain four arrays simultaneously.
3. A GeneChip Scanner 3000 7G for obtaining high-resolution images of hybridization signals. The scanner can scan 64 array simultaneously.
4. A GeneChip Workstation that controls the operation of the system, data collection, and processing of initial raw data.
5. A bioinformatics system, including Expression Console, Transcriptome Analysis Console, and Ingenuity Pathway Analysis.