The Research Core Facility at LSU Health Shreveport provides investigators, post-doctoral fellows, and graduate and undergraduate students with access to a wide variety of research services, educational/training opportunities and instrumentation.

Located on the 6th floor of the Biomedical Research Institute, each technology is staffed by trained research specialists.  These technologies include flow cytometry, laser capture microdissection, mass spectrometry, microarray, fluorescence, confocal, and super resolution confocal microscopy, next generation sequencing, and real-time PCR. The Research Core Facility supports both the experienced and novice investigator.

We encourage you to explore our facility as well as other Core Facilities on our campus.

Microscopy

Leica TCS SP5 Spectral Confocal Microscope

Nikon A1R Confocal & N-SIM Super Resolution System

Nikon Eclipse TE300 Widefield Microscope

Zeiss AxioObserver Z1 Widefield/Apotome Microscope
 
Zeiss LSM 510 NLO Confocal/Multiphoton Microscope

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Flow Cytometry

BD Biosciences FACSAriaIII Flow Cytometer/Cell Sorter

BD Biosciences FACSCalibur Flow Cytometer

BD Biosciences LSRII Flow Cytometer

BD Biosciences LSRII-SORP Flow Cytometer

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Genomics

Microarray
Affymetrix GeneChip System

Laser Capture Microdissection
Arcturus XT LCM System 

Next Generation Sequencing
Illumina MiSeq Sequencer
Illumina NextSeq 550 Sequencer

Real-Time Quantitative PCR
Bio-Rad CFX96 Fast Real-Time PCR Instruments (3)
Bio-Rad CFX384 Touch Real-Time PCR Instrument

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Mass Spectrometry

Thermo Finnigan Deca XP Max Nanospray LC/MS Mass Spectrometer

Waters SYNAPT High-Resolution Mass Spectrometer

Waters Q-TOF Micro Mass Spectrometer

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Equipment Description & Usage

MICROARRAY

Scientific Advisor: Dr. Rona Scott
Genomics Research Specialist: Camille Abshire, MS, MDXT-(AAB), CLS (LSBME)

Affymetrix GeneChip System
This system is suitable for global gene expression studies using the Affymetrix GeneChip Probe arrays. Oligonucleotide arrays, prepared on glass, are hybridized to biotinylated probes prepared from biological samples and detected with a fluorescent label. Probes for these experiments are derived from a single source, and differentially expressed genes are identified by comparing the results of experiments performed with different chips. A major advantage of this approach is the ready availability of pre-prepared arrays representing a large number of sequences from a number of species.
This system consists of the following components:
1.    A GeneChip Hybridization Oven 640 for automated control of hybridization to the GeneChip arrays.
2.    A GeneChip Fluidics Station 450 for automated washing of chips and labeling of hybridized probes. This station can wash and stain four arrays simultaneously.
3.    A GeneChip Scanner 3000 7G for obtaining high-resolution images of hybridization signals. The scanner can scan 64 array simultaneously.
4.    A GeneChip Workstation that controls the operation of the system, data collection, and processing of initial raw data.
5.    A bioinformatics system, including Expression Console, Transcriptome Analysis Console, and Ingenuity Pathway Analysis.

CONTACT US

Kelly Tatchell, PhD
Director, Research Core Facility
Associate Dean of Graduate Studies
ktatch@lsuhsc.edu
(318) 675-7769

Paula Polk, MS
Assistant Director, Research Core Facility
ppolk@lsuhsc.edu
(318) 675-4939

Camille Abshire, MS, MDxT-(AAB), CLS (LSBME)
Research Specialist, Genomics
cabsh2@lsuhsc.edu
(318) 675-4174

David Custis, MS
Research Specialist, Flow Cytometry
dcusti@lsuhsc.edu
(318) 675-4174

Chaowei Shang, PhD
Research Specialist, Microscopy
cshang@lsuhsc.edu
(318) 675-8537

Xinggui Shen, PhD
Mass Spectroscopy Specialist
Assistant Professor, Pathology
xshen@lsuhsc.edu
(318) 675-3371

Ana-Maria Dragoi, MD, PhD
Associate Director, INLET
Feist-Weiller Cancer Center 
adrag1@lsuhsc.edu
(318) 675-4216

Jennifer Carroll, PhD
Translational Research Specialist, INLET
Feist-Weiller Cancer Center 
jcarro1@lsuhsc.edu
(318) 675-4234

Research Core Facility

Research Core Facility

Research Core Facility

Research Core Facility