The Research Core Facility at LSU Health Shreveport provides investigators, post-doctoral fellows, and graduate and undergraduate students with access to a wide variety of research services, educational/training opportunities and instrumentation.

Located on the 6th floor of the Biomedical Research Institute, each technology is staffed by trained research specialists.  These technologies include flow cytometry, laser capture microdissection, mass spectrometry, microarray, fluorescence, confocal, and super resolution confocal microscopy, next generation sequencing, and real-time PCR. The Research Core Facility supports both the experienced and novice investigator.

We encourage you to explore our facility as well as other Core Facilities on our campus.

Microscopy

Leica TCS SP5 Spectral Confocal Microscope

Nikon A1R Confocal & N-SIM Super Resolution System

Nikon Eclipse TE300 Widefield Microscope

Zeiss AxioObserver Z1 Widefield/Apotome Microscope
 
Zeiss LSM 510 NLO Confocal/Multiphoton Microscope

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Flow Cytometry

BD Biosciences FACSAriaIII Flow Cytometer/Cell Sorter

BD Biosciences FACSCalibur Flow Cytometer

BD Biosciences LSRII Flow Cytometer

BD Biosciences LSRII-SORP Flow Cytometer

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Genomics

Microarray
Affymetrix GeneChip System

Laser Capture Microdissection
Arcturus XT LCM System 

Next Generation Sequencing
Illumina MiSeq Sequencer
Illumina NextSeq 550 Sequencer

Real-Time Quantitative PCR
Bio-Rad CFX96 Fast Real-Time PCR Instruments (3)
Bio-Rad CFX384 Touch Real-Time PCR Instrument

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Mass Spectrometry

Thermo Finnigan Deca XP Max Nanospray LC/MS Mass Spectrometer

Waters SYNAPT High-Resolution Mass Spectrometer

Waters Q-TOF Micro Mass Spectrometer

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Equipment Description & Usage

MASS SPECTROMETRY

Scientific Advisors: Dr. Chris Kevil and Dr. Hyung Nam
Technical Staff:  Dr. Xinggui Shen

Waters SYNAPT High-Resolution Mass Spectrometer
This instrument is capable of operating in both quadrupole time-of-flight (Q-TOF) and ion mobility mode and is primarily used for unbiased detection of peptides and metabolites for proteomics and metabolomics applications, respectively. A NanoAcquity UPLC system with 2D technology is directly interfaced to nanospray ionization source of SYNAPT HDMS for proteomics applications.  An Acquity UPLC system is interfaced to ESI/atmospheric pressure chemical ionization (APCI) source for metabolomics applications.  An atmospheric pressure photoionization (APPI) source is also available for Synapt. 

Waters Q-TOF Micro
This is a quadrupole/time-of-flight mass spectrometer for MS/MS-based sequencing of peptides with a mass resolving power of ~8,000FWHM.  The Q-TOF is interfaced to a Waters CapLC HPLC system with integrated auto sampler for automated unattended nLC-MS/MS.  Determination of peptide sequences from MS/MS spectra will be through a suite of software tools including ProteinLynx Global Server (2.2), SEQUEST and MASCOT. 

Thermo Finnigan Deca XP Max Nanospray LC/MS Mass Spectrometer
This ion trap mass spectrometer is capable of extremely rapid MS/MS experiments.  Ultra-high sensitivity coupled with high resolution and fast scan speeds allows for exceptional peptide sequence coverage and metabolites quantification. This instrument is utilized for sensitive bio molecular identification and experiments requiring MSn.  It is coupled to a Michrom Paradigm MG4 multidimensional LC system with integrated auto sampler.

CONTACT US

Kelly Tatchell, PhD
Director, Research Core Facility
Associate Dean of Graduate Studies
ktatch@lsuhsc.edu
(318) 675-7769

Paula Polk, MS
Assistant Director, Research Core Facility
ppolk@lsuhsc.edu
(318) 675-4939

Camille Abshire, MS, MDxT-(AAB), CLS (LSBME)
Research Specialist, Genomics
cabsh2@lsuhsc.edu
(318) 675-4174

David Custis, MS
Research Specialist, Flow Cytometry
dcusti@lsuhsc.edu
(318) 675-4174

Chaowei Shang, PhD
Research Specialist, Microscopy
cshang@lsuhsc.edu
(318) 675-8537

Xinggui Shen, PhD
Mass Spectroscopy Specialist
Assistant Professor, Pathology
xshen@lsuhsc.edu
(318) 675-3371

Ana-Maria Dragoi, MD, PhD
Associate Director, INLET
Feist-Weiller Cancer Center 
adrag1@lsuhsc.edu
(318) 675-4216

Jennifer Carroll, PhD
Translational Research Specialist, INLET
Feist-Weiller Cancer Center 
jcarro1@lsuhsc.edu
(318) 675-4234

Research Core Facility

Research Core Facility

Research Core Facility

Research Core Facility