Nikon TE300 Widefield

The system is built around a Nikon Eclipse TE300 inverted microscope with a range of objectives for phase, DIC, and high resolution epi-fluorescent imaging. Software packages control a Prior OptiscanTM xyz stage with a full complement of stage inserts and a Prior filter wheel containing excitation filters from the Chroma 83000 filter set. This set includes single and multiband excitation filters for DAPI, FITC, GFP, Texas Red, Rhodamine, or PI. Images are acquired with either a monochrome CCD camera, or a colored camera for brightfield images.

Reservation Calendar

Capability

Phase contrast, DIC, and bright-field imaging (colored-camera)
Multichannel fluorescent imaging (black and white camera)
Multipoints, large image scan (Mosaix)

Sample

Coverslip thickness: No. 1.5 (equals to 0.17 mm)
Plastic plates and dishes can also be used in addition to glass bottom ones (some objectives are designed for plastic plates/dishes).

Objectives

Objectives

Name

Immersion

Location

4x/0.20 NA

Plan Apo WD 20

Air

Microscope

10x/0.30 NA

Plan Fluor Ph1 DLL

Air

Microscope

20x/0.45 NA

Plan Fluor ELWD DIC L

Air

Microscope

20x/0.50 NA

Plan Fluor Ph1 DLL

Air

Microscope

40x/0.60 NA

Plan Fluor ELWD DIC M

Air

Microscope

40x/1.30 NA

Plan Fluor DIC H

Oil

Microscope

60x/1.40 NA

Plan Apo DIC H WD 0.21

Oil

Cabinet

60x/1.40 NA

Plan Apo VC DIC N2

Oil

Cabinet

60x/1.40 NA

Plan Apo VC DIC N2

Oil

Cabinet

100x/1.40 NA

Plan Apo DIC H WD 0.13

Oil

Cabinet

CONTACT US

Chaowei Shang, PhD
Research Specialist, Microscopy
cshang@lsuhsc.edu
(318) 675-8537

 

Hungwen (Kevin) Lin, PhD
Scientific Adviser, Microscopy
Assistant Professor, Neurology
hlin2@lsuhsc.edu
(318) 675-8810