Biomechanics is especially important in Orthopaedic Surgery, a specialty that requires precision to treat injuries relating to bones, joints, tendons, muscles and other aspects of the body’s musculoskeletal system. LSU Health Shreveport faculty physicians, medical students, residents and researchers are teaming up in the Biomechanics Laboratory to assist Orthopaedic surgeons in the development and evaluation of surgical approaches.
Giovanni Solitro, PhD, Assistant Professor of Orthopaedic Surgery, directs the Biomechanics Lab at LSU Health Shreveport. The two personnel who attend to the lab include a Research Technician and Research Associate with a combined 50 years of Orthopaedic Research related activities. Teams in the lab consist of four people: Dr. Solitro, a faculty physician, a resident, and a medical student.
The only known lab of its kind in Louisiana, the Biomechanics Lab at LSU Health Shreveport provides the environment, equipment, and tools for research within the basic science aspect of Orthopaedics. The lab is completely independent and has control over the research project processes from concept to prototype.
Advancement in Orthopedic science is obtained through the analysis of surgical procedures in simple, mathematically driven tasks, and the development of surgical tools for procedures when this analysis is too difficult to be performed in real time. Two research methods are used develop and evaluate a wide array of Orthopaedic surgical approaches: in silico experiments, which are conducted or produced with computer modeling or computer simulation, and in vitro experiments, which take place in the lab with the assistance of specialized equipment.
Computer Aided Engineering (CAE) and Computer Aided Manufacturing (CAM) software programs are critical to the lab’s work. The 720 sq. ft. lab houses Instron bi-axial testing frames. Fabrication equipment, which allows the team the capability to create their own customized testing equipment, includes machining tools and a multi-axis CNC milling machine.
The lab is currently working on several projects with the Orthopaedic Residents and involves LSUHS Medical and LATech Bioengineering students.
The reach of the Biomechanics Lab goes beyond LSU Health Shreveport as students from the Bossier Parish Community College (BPCC) engineering prep program are participating in a non-credit biomechanics internship. This internship allows students access to a biomechanics lab, which is not an available resource at BPCC.
Tissue Regeneration Research
Dr. Yufeng Dong, MD, PhD is involved in cellular research related to skeletal tissue regeneration, molecular mechanisms involving osteoarthritis, and developing translational solutions to develop cell based cartilage and repair of bone defects and fracture repairs.
- The molecular and cellular events underlying stem cell differentiation that leads to skeletal tissue regeneration, including Notch, Wnt and TGF beta signaling
- The molecular mechanism underlying human osteoarthritis.
- An emphasis on challenging clinical problems and translational solutions, including stem cell based cartilage, bone defect and fracture repair.
- Human and mouse bone marrow stem cell isolation and culture
- Osteoblast and chondrocyte culture and differentiation
- Mouse femur bone defect and tibia fracture surgery
- Induced stem cell osteogenic and chondrogenic differentiation assay
- Mouse limb bud stem cell isolation and micromass culture
- Real time PCR for gene expression in tissue and cells
- Flow Cytometry for cell sorting and surface marker assay
- Bone and cartilage tissue histological analysis
- Cell sheet culture and transplantation
- Cells and biomaterials ectopic bone formation in vivo assay
Sheila Rogers, Scientific Research Technologist
Dollie Smith, Research Associate
Bo Tian, Postdoctoral fellow
Junkui Sun, Postdoctoral fellow
Xiaoya Xu, Postdoctoral fellow
Guangxi Wang, Postdoctoral fellow
Emily Barsley, Medical student
Rachel Lee, Medical student
Jordan Hanberry, Medical student
Clay Rodriguez, Medical student