Translational Research

Research Interests

Abuse of opioid drugs is a growing public health problem in the United States. In addition to classical opiates such as heroin and morphine, there have been dramatic increases in the nonmedical use of prescription opioids, such as oxycodone and hydrocodone. Recently, the correlation between opioid analgesics and alternations in brain structure, such as leukoencephalopathy, axon demyelination, and white matter lesions, has been demonstrated in patients with a history of long-term use of prescription opioids. Ours and other's studies indicate that the opioids alter gene expression and signaling pathways in different regions of the brain. The research of our group is focused on mechanisms of opioid-induced neuronal degeneration using animal and cell culture models as well as biomedical methods. Data from our studies suggest that aberrant translation mechanism, called integrated stress response, contributes to opioid-induced neuronal degeneration using blood samples. Such method could be used to detect toxic side effects of opioids and other medications and monitor progression of aging and neurodegenerative diseases. In addition, in collaboration with computer scientists from LSU-S, we analyze trends in drug abuse among hospital visitors using large data analysis.

Methods Used

  • Animal models of drug abuse (rats, mice)
  • Mammalian cell culture
  • Western blot analysis, IHC, IFC
  • Polysomal profiling of mRNA, q-RT-PCR
  • Cloning
  • Expression and purification of recombinant proteins
  • In-vitro transcription/translation

Ongoing Projects

  • Opioid-induced neuronal degeneration: implication of the integrated stress response
  • Role of the protein synthesis signaling in drug use disorders
  • Trends in Demographic and Drug Screen Results among LSUHSC-Shreveport Emergency Department patient visits

Recent Publications

Substance Abuse. Elyse M. Cornett, Rebecca Budish, Nadejda Korneeva, Mark Jones. (2019) Chapter in the Springer Nature Switzerland AG 2019 book. Charles J. Fox, Elyse M. Cornett, G. E. Ghali (eds.), Catastrophic Perioperative Complications and Management. A Comprehensive Textbook. DOI: 10.1007/978-3-319-96125-5_3

GatewayNet: A Form of Sequential Rule Mining. Phillip CSR Kilgore, Nadejda Korneeva, Thomas C. Arnold, Marjan Trutschl, and Urska Cyek. (2019) BMC Medical Informatics and Decision Making. Apr 23; 19(1):87. PMID: 31014328

Chronic Opioids Induce Axonal Degeneration in Rat Brain. Ruping Fan, Lisa M Schrott, Thomas Arnold, Stephen Snelling, Meghana Rao, Derrel Graham, Angela Cornelius, and Nadejda L Korneeva. (2018) BMC Neurosci. Mar 23;19(1):15. PMID: 29571287

Urine Drug Screen Trends from 1998 to 2011 Among Emergency Department Patients Treated in a University Teaching Hospital. Korneeva N, Cyek U, Leskova A, Hutchinson K, Callahan A, Patek G, Trutschl M, Kilgore PCSR, McGauly P, Goeders N, Arnold T. (2018) Toxicology Communications. 2(1):24-34. PMID: 30906915

Chronic Oxycodone Induces Integrated Stress Response in Rat Brain. ruping Fan, Lisa M. Schrott, Stephen Snelling, Julius Ndi, Thomas Arnold, Nadejda L Korneeva. (2015) BMC Neuroscience. 16:58. DOI: 10.1186/s12868-015-0197-8. PMID: 26377394

Emergency Medicine Research