Zeiss LSM Confocal & Two-Photon System

The Zeiss LSM 510 NLO system is configured to enhance living tissue research. The scanning system is connected to an upright Axioskop 2 FS MOT microscope with a set of objectives selected for physiological measurements and live animal studies. The stage remains in a fixed position, and the objectives have motorized focus control.

 

Reservation Calendar

Capability

Fixed sample and live animal imaging
Bright field and fluorescence imaging
Two-photon fluorescence imaging
2D, 3D, and 4D image collection and processing, 3D/4D animation, calibration and measurement of ion concentration, time series analysis, and graphical mean ROI analysis.
 

Lasers

Argon (458, 477, 488, 514 nm),
HeNe (543nm),
HeNe (633nm),
Coherent Chameleon-XR Ti:Sapphire laser (tunable from 705 through 980nm).

Samples

The ultrafast pulsed Chameleon laser emitting NIR radiation allows imaging up to 500 μm deep within tissue.
Tissue slides may reach 1 mm thick.

Objectives

Obj

Objective

Medium

10x

EC Plan-Neofluar 10x/0.3

Dry

20x

Achroplan 20x/0.5 W

Water

20x

LD Plan-Neofluar 20x/0.4 Ph2 Korr

Dry

25x

LCI Plan-Neofluar 25x/0.8 oil DIC ∞0-0.17 Glyc-w Imm Korr

Glycerin and Water

40x

W Plan-Apochromat 40x/1.0 DIC VIS-IR

Water

40x

C-Apochromat 40x/1.2 W Korr UV-VIS-IR ∞0.14-0.19

Water

40x

IR-Achroplan 40x/0.80 W

Water

63x

IR-Achroplan 63x/0.90 W

Water

63x

W Plan-Apochromat 63x/1.0 VIS-IR

Water

63x

Plan-Apochromat 63x/1.4 oil DIC ∞/0.17

Oil

Detectors

Three PMTs for visible wavelength detection
A transmitted light detector
Two non-descanned (ND) detectors for multiphoton imaging.

Software

The operating software is ZEN 2009 that permits 2D, 3D, and 4D image collection and processing, 3D/4D animation, calibration and measurement of ion concentration, time series analysis, and graphical mean ROI analysis.

CONTACT US

 

Chaowei Shang, PhD
Research Specialist, Microscopy
cshang@lsuhsc.edu
(318) 675-8537

 

Hungwen (Kevin) Lin, PhD
Scientific Adviser, Microscopy
Assistant Professor, Neurology
hlin2@lsuhsc.edu
(318) 675-8810