Zeiss AxioObserver & Apotome

The system is built with a Zeiss Axio Observer Z1 inverted fluorescent microscope, fully automated with component recognition to minimize errors. System components include mercury arc lamp excitation, a Zeiss AxioCamMRm CCD camera with 12-bit dynamic range and extended sensitivity in the near infrared, fully automated xyz stage, a complement of objectives from 10x through 100x, 5 installed filter sets for DAPI, FITC, narrow band GFP, Rhodamine, and far red. The Apotome attachment is designed for precise optical sectioning. The Apotome projects a grid onto the image plane, which allows 3 raw images taken. A software algorithm then removes out-of-focus signals based on the 3 raw images.

Reservation Calendar

Capability

Bright field (Phase contrast, DIC)
Multichannel fluorescence
Z-stack, Mark & Find, Mosaic (image stitching), and Time lapse
Inside 4D, Colocalization

Sample Size

Apotome optical sectioning: Best for samples no more than 20 microns thick.

Objectives

Obj Objective Medium Grid
10x EC Plan-Neofluar 10x/0.3 DIC I Air L
20x Plan-Apochromat 20x/0.8 DIC II Air L
40x LD Plan-Neofluar 40x/0.6 Ph2 DIC II Air NA*
40x Plan-Apochromat 40x/1.3 DIC (UV) VIR-IR Oil L
63x Plan-Apochromat 63x/1.4 DIC III Oil H
100x Plan-Apochromat 100x/1.4 Oil D

Filters

Cube Turret Position Ex Filter Dichroic (FT) Em Filter Quote Name
Brightfield 1        
34 2 390/22 420 460/50 BLUE GFP
38 HE 3 470/40 495 525/50 EGFP
31 4 565/30 585 620/60 Cy 3.5
17 5 485/20 510 515/565 BP
50 6 640/30 660 690/50 Cy 5

 

 

CONTACT US

 

Chaowei Shang, PhD
Research Specialist, Microscopy
cshang@lsuhsc.edu
(318) 675-8537

 

Hungwen (Kevin) Lin, PhD
Scientific Adviser, Microscopy
Assistant Professor, Neurology
hlin2@lsuhsc.edu
(318) 675-8810