Nikon A1R Confocal & Super Resolution System

This is a high-speed A1R confocal microscope combined with a SIM super resolution system. The Nikon Eclipse Ti-E inverted microscope is built with fully automated objectives, stage, and filter turrets. The microscope is housed in an incubator with CO2, O2, and humidity control.


Reservation Calendar

Capabilities

Fixed and live sample imaging
Bright-field, phase contrast, and fluorescence imaging
FRET (Fluorescence Resonance Energy Transfer)
FRAP (Fluorescence Recovery After Photobleaching)
Multipoint acquisition, Multichannel acquisition, Large image scan (image stitching), Z-stack, and 2D/3D deconvolution.

Strength of SIM

The lateral resolution of the super resolution system can reach to 85-115nm, and the axial resolution can reach to 270nm. This doubles the resolution of a conventional confocal microscope.

PFS: Perfect Focus System with a 3 mm range to combat focal drift and maintain long term time-lapse focus stability.

Lasers

405nm, 488nm, 561nm, 640nm
AOTF (Acousto-Optical Tunable Filter) installed

Detectors

Transmitted light detector
Two GasApe detectors (for lasers 488 and 561)
Two standard detectors (for lasers 405 and 640)

 

Objectives

Obj

Objective

Medium

Use

10x

Plan Apo 10x/0.45NA

Dry

Confocal

20x

Plan Apo VC 20x/0.75NA  DIC

Dry

Confocal

40x

Plan Fluor 40x/1.30NA  DIC

Oil

Confocal

60x

Apo 60x/1.40NA DIC

Oil

Confocal

100x

SR Apo TIRF 100x/1.49NA 

Oil

Confocal/SIM

Samples

Confocal optical sectioning is best for samples less than 100 microns thick.

SIM imaging is best for samples less than 20 microns thick
Deconvolution is best for samples no more than 5 microns thick.

Software

Nikon NIS-Elements C software in a Windows 10 operating system

CONTACT US

 

Chaowei Shang, PhD
Research Specialist, Microscopy
cshang@lsuhsc.edu
(318) 675-8537

 

Hungwen (Kevin) Lin, PhD
Scientific Adviser, Microscopy
Assistant Professor, Neurology
hlin2@lsuhsc.edu
(318) 675-8810