Leica TCS SP5 Confocal

This is a very flexible and fast confocal system build with a Leica DMI 6000 CS inverted, fully automated microscope with motorized stage, condenser, objective and filter turrets. The microscope is housed in a Ludin full enclosure incubator with an internal Ludin Cube2 with CO2, O2, and humidity control.
 

Reservation Calendar

Capability

Fixed and living sample imaging
Bright field, phase contrast, and fluorescence imaging
FRAP (Fluorescence Recovery After Photobleaching )
FRET (Fluorescence Resonance Energy Transfer)
Mark & Find, Tile Imaging (image stitching), Z-stack, 3D Visualization, Co-localization

Sample

Optical sectioning best for samples no more than 100 microns thick

Lasers

blue diode (405nm)
Multi-line argon (458,476,488,496,514nm)
Green HeNe (543nm)
Orange HeNe (594nm)
Red HeNe (633nm)

Objectives 

Obj

Objective

Medium

 

2.5x

PL FLUOTAR 2.5x/0.07NA

Air

Available

10x

HC PL APO 10x0.40NA

Air

Installed

20x

HC PL APO 20x/0.7NA Imm Corr

Air

Installed

40x

HCX PL APO CS 40x/0.75U-V-I 0.17

Air

Installed

40x

HCX PL APO CS 40x1.24-0.75NA

Oil

Installed

63x

HCX PL APO CS 63x/1.4-0.6NA

Oil

Installed

63x

HCX PL APO CS 63x/1.3NA glycerol corrected

Glycerol

Available

100x

HCX PL APO CS 100x/1.46

Oil

Installed

100x

HCX PL APO CS 100x/1.40-0.70NA

Oil

Available

Detectors

Transmitted light detector
Spectral beam splitter with freely adjustable bandwidth and 5 PMT (photomultiplier detectors)

Software

LAS AF SP5 acquisition software
Three LAS AF SP5 offline software packages with 3D analysis, multicolor analysis, microlabe and live data mode

CONTACT US

Chaowei Shang, PhD
Research Specialist, Microscopy
cshang@lsuhsc.edu
(318) 675-8537

 

Hungwen (Kevin) Lin, PhD
Scientific Adviser, Microscopy
Assistant Professor, Neurology
hlin2@lsuhsc.edu
(318) 675-8810