Mass Spectrometry

The mission of the Mass Spectrometry Core in the LSU Health-Shreveport Research Core Facility is to provide efficient and cost effective proteomics- and metabolomics-based services for research investigators.

The facility is located in room F6-12 in the Biomedical Research Institute. For protein analysis, our principal approach is bottom-up proteomics, which is a common method to identify proteins and characterize their amino acid sequences and post-translational modifications by proteolytic digest prior to analysis by mass spectrometry. In addition to our standard services, we also strive to continue to develop new, state-of-the-art mass spectrometry-based analyses based upon input and specific requests from our users.

Instrumentation

LCQ-DECA XP

The LCQ-DECA XP is an ion-trap mass spectrometer with a dedicated Thermo HPLC system for highly selective and sensitive detection of metabolites and peptides. The instrument’s ion trap mass analyzer is particularly useful in cases where the molecular mass of the analyte is completely unknown. While enabling MSn capabilities, the ion trap provides structural information, allowing the analyst to “Top-down” the structure of the molecule. It is coupled to a Michrom Paradigm MG4 multidimensional LC system (Thermo) with integrated auto sampler. Finally, data dependent scanning allows the user to identify protein/peptide modifications.

Q-TOF Micro

This is a quadrupole/time-of-flight mass spectrometer for MS/MS-based sequencing of peptides with a mass resolving power of ~8,000FWHM. The Q-TOF is interfaced to a Waters CapLC HPLC system with integrated auto sampler for automated unattended nLC-MS/MS. The Q-TOF mass analyzer is particularly useful for the identification of post-translational modification.

SYNAPT HDMS

This instrument is capable of operating in both quadrupole time-of-flight (Q-TOF) and ion mobility mode and is primarily used for unbiased detection of peptides and metabolites for proteomics and metabolomics applications, respectively. A nanoAcquity UPLC system with 2D technology is directly interfaced to nanospray ionization source of SYNAPT HDMS for proteomics applications. An Acquity UPLC system is interfaced to ESI/atmospheric pressure chemical ionization (APCI) source for metabolomics applications.  An atmospheric pressure photoionization (APPI) source is also available for the Synapt HDMS. 

XEVO TQ

This instrument is a tandem quadrupole mass spectrometer with a dedicated acquity UPLC system for highly selective and sensitive detection of metabolites and peptides. Furthermore, ScanWave technology of Xevo TQ provides enhanced spectral LC/MS/MS data acquisition capabilities.

Fees & Services

Core Services

Pricing

In-solution digestion

$40

In-gel digestion (per spot or band)

$40

FFPE protein extraction

$30

Solid phase extraction (C18, HLB)

$20

Concentrating and purifying peptide by Zip tips

$10

Amicon MW cutoff filtration

$20

TiOenrichment for phosphopeptides

$30

Antibody-based enrichment (not including antibody reagent costs)

$40

Protein ID by LC-MS/MS 60 min

$55

Protein ID by 2D LC-MS/MS 5 fraction

$400

Protein ID by 2D LC-MS/MS 10 fraction

$800

Custom database searches by PEAKS software

$5/hour

Additional data analysis

Contact


Please note: The prices above are effective through December 21, 2019, and are based on an a la carte menu of services currently utilized. To obtain an estimate of the cost for your specific project, please consult with Dr. Xinggui Shen.

Sample Submission

Three steps are required to submit a sample to our facility:

  1.  Prior to submitting a sample, please arrange for a consultation with MS Core Staff. Samples will not be accepted without prior consultation.
     
  2. A sample submission form will need to be filled out and submitted it to Dr. Xinggui Shen (xshen@lsuhsc.edu).  Please download the sample submission here.
     
  3. After your sample has been assigned a PROJECT NUMBER, the MS Core staff will manage the project and will conduct the requested analysis based on established Standard Operating Procedures.

User Guidelines

The Mass Spectrometry Core was established to provide services to the biomedical research community at LSU Health-Shreveport. Although samples will be run by Core staff, we expect our users to familiarize themselves with sample preparation, data acquisition and interpretation and be an active part of sample analysis.

Publication Acknowledgement

For all publications that include data generated in the Mass Spectrometry Core, we kindly request that you acknowledge this support in the following manner:

We thank the Research Core Facility Mass Spectrometry Core at LSU Health-Shreveport, which is supported in part by the LSU Health Shreveport Foundation, the Biomedical Research Foundation, the Center for Cardiovascular Diseases and Sciences, and the Feist-Weiller Cancer Center.

CONTACT US

Xinggui Shen, PhD
Technical Staff
Assistant Professor, Pathology
xshen@lsuhsc.edu
(318) 675-3371

 

Christopher Kevil, PhD
Scientific Advisor, Mass Spectrometry
Vice Chancellor for Research and Dean, School of Graduate Studies
ckevil@lsuhsc.edu
(318) 675-4694

 

Hyung Nam, PhD
Scientific Advisor, Mass Spectrometry
Assistant Professor, Department of Pharmacology & Toxicology
hnam@lsuhsc.edu
(318) 675-3241