Cellomics ArrayScan V
ArrayScan VTI HCS Reader is an automated fluorescent microscopic imaging system designed for high content screening in fixed or live cells. The system is also equipped with a live cell chamber for acquisition of cells in temperature, humidity, and CO2 controlled environment.
- Fully automated microscope optics by Carl Zeiss
- Automated Stage can accommodate 96 or 384 well micro-plate or slides
- High-resolution, shutterless cooled CCD camera
- Automated objective changer (10X, 20X, 40X, 63X)
- HCS studio software
- LED light source
- Excitation wavelengths:
- 386/23 (DAPI, Hoechst)
- 485/20 (FITC, GFP, YFP, Alexa Fluor® 488)
- 549/15 (TRITC, RFP, Alexa Fluor® 594)
- 650/13 (Cy5, Alexa Fluor® 647)
The Cellomics ArrayScan VTI high-content analysis system complements the high-throughput resources in the Screening Core. This platform allows for both drug screening with over 30 different application based cell phenotypic assays as well as for high throughput research purposes. INLET has upgraded the Cellomics high content imaging platform with the purchase of a new high resolution CCD camera, a Dell Precision workstation and HCS studio software upgrade.
The new X1 CCD camera has significantly improved quantum efficiency by four times the previous camera especially, in wavelengths greater than 550 nm. The X1 CCD camera is more sensitive to lower energy wavelengths, and increases the biological field of view/capture by 50%. Both the 40X and 63X objectives with high numerical apertures allows us to visualize submicron sizes of cellular objects and definitively view fragmented compartments. Having the upgrades to our system has improved our functionality as a core by opening a multitude of possibilities in imaging subcellular structures in mammalian cells and in yeast.
HCS Studio Cell Analysis Software allows access to all instrument configuration and control functions with build-in tools. The user can select from a menu of more than 30 pre-established bioapplications that can be optimized to cell-line and specific phenotype or can create a new assay protocol using the tools and design of HCS Studio Cell Analysis Software.
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