The Research Core Facility at LSU Health Shreveport provides investigators, post-doctoral fellows, and graduate and undergraduate students with access to a wide variety of research services, educational/training opportunities and instrumentation.

Located on the 6th floor of the Biomedical Research Institute, each technology is staffed by trained research specialists.  These technologies include flow cytometry, laser capture microdissection, mass spectrometry, microarray, fluorescence, confocal, and super resolution confocal microscopy, next generation sequencing, and real-time PCR. The Research Core Facility supports both the experienced and novice investigator.

We encourage you to explore our facility as well as other Core Facilities on our campus.

Microscopy

Leica TCS SP5 Spectral Confocal Microscope

Nikon A1R Confocal & N-SIM Super Resolution System

Nikon Eclipse TE300 Widefield Microscope

Zeiss AxioObserver Z1 Widefield/Apotome Microscope
 
Zeiss LSM 510 NLO Confocal/Multiphoton Microscope

Learn more

Flow Cytometry

BD Biosciences FACSAriaIII Flow Cytometer/Cell Sorter

BD Biosciences FACSCalibur Flow Cytometer

BD Biosciences LSRII Flow Cytometer

BD Biosciences LSRII-SORP Flow Cytometer

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Genomics

Microarray
Affymetrix GeneChip System

Laser Capture Microdissection
Arcturus XT LCM System 

Next Generation Sequencing
Illumina MiSeq Sequencer
Illumina NextSeq 550 Sequencer

Real-Time Quantitative PCR
Bio-Rad CFX96 Fast Real-Time PCR Instruments (3)
Bio-Rad CFX384 Touch Real-Time PCR Instrument

Learn more

Mass Spectrometry

Thermo Finnigan Deca XP Max Nanospray LC/MS Mass Spectrometer

Waters SYNAPT High-Resolution Mass Spectrometer

Waters Q-TOF Micro Mass Spectrometer

Learn more

 

RCF Equipment Descriptions

FLOW CYTOMETRY 
Scientific Advisor: Dr. Matthew Woolard
Flow Cytometry Research Specialist: David Custis, MS


BD Biosciences FACSAriaIII
The FACSAriaIII cell sorter is capable of 17-parameter (Forward Scatter, Side Scatter, and 15 fluorescence detectors) analysis and cell sorting. It has four solid-state lasers for excitation at wavelengths of 405 nm, 488 nm, 561 nm and 633 nm. This is a digital high-speed sorter, capable of sorting up to 70,000 events per second. It is capable of standard “bulk” sorting of up to four user-defined cell populations simultaneously, or can be used for direct deposition of a counted number of cells directly into tissue culture plates for cloning, frequency response assays, or other single cell analyses. This instrument uses BD Biosciences FacsDiva software for acquisition and analysis.

BD Biosciences FACSCalibur
The RCF maintains a FACSCalibur flow cytometer available for investigator use.  Documented training by staff is required for its use.  The FACSCalibur is an ultra-sensitive flow cytometer, capable of six parameter (two laser light scatter and up to four fluorescent colors) analysis.  It uses two lasers for fluorochrome excitation: an Argon ion laser for 488 nm excitation and a Red Diode laser for 635 nm excitation. This instrument uses CellQuest Pro software for acquisition and analysis.

BD Biosciences LSRII-UV
This LSRII is capable detecting up to 14 parameters (Forward Scatter, Side Scatter, and twelve fluorescence detectors).  It has four solid-state lasers for excitation at wavelengths of 355 nm, 405 nm, 488 nm, and 633 nm.  This instrument uses BD Biosciences FacsDiva software for acquisition and analysis.

BD Biosciences LSRII-SORP
This LSRII is capable of detecting up to 17 parameters (Forward Scatter, Side Scatter, and fifteen fluorescence detectors).  It has four solid-state lasers for excitation at wavelengths of 405 nm, 488 nm, 561 nm and 633 nm.  This instrument uses BD Biosciences FacsDiva software for acquisition and analysis.

Flow Cytometry Workstations and Software
There is a Macintosh MacPro and two Dell PCs for off-line analysis of data. The off-line Macintosh computer is loaded with Cellquest Pro, while the off-line PCs are loaded with the latest version of FacsDiva software and FCS Express for data analysis. In addition, FlowJo is available on the Macintosh and Dell workstations for specific data analysis needs. For the analysis of cell cycle data, ModFit LT is available on both the Macintosh and PC workstations.

LASER CAPTURE MICRODISSECTION
Scientific Advisor: Dr. Rona Scott
Genomics Research Specialist: Camille Abshire, MS, MDXT-(AAB), CLS (LSBME)

Arcturus XT
The Arcturus XT instrument performs Laser Capture Microdissection from heterogeneous tissue samples simply, quickly, and precisely. In minutes the investigator can locate a single cell or large groups of cells and, using a simple aim-and-shoot method, extract them for subsequent molecular analysis. LCM preserves the exact morphologies of both the captured cells and the surrounding tissue. The Arcturus XT transfers cells from paraffin-embedded and frozen tissue sample stained slides. The entire process can be monitored and documented, and the images stored in an archiving workstation.  Research applications include genomics (differential gene profiling, loss of heterozygosity, microsatellite instability, and gene quantification) and proteomics (two-dimensional protein gels, Western blotting, and immuno-quantification of proteins).  

MASS SPECTROMETRY
Scientific Advisors: Dr. Chris Kevil and Dr. Hyung Nam
Technical Staff:  Dr. Xinggui Shen

Waters SYNAPT High-Resolution Mass Spectrometer
This instrument is capable of operating in both quadrupole time-of-flight (Q-TOF) and ion mobility mode and is primarily used for unbiased detection of peptides and metabolites for proteomics and metabolomics applications, respectively. A NanoAcquity UPLC system with 2D technology is directly interfaced to nanospray ionization source of SYNAPT HDMS for proteomics applications.  An Acquity UPLC system is interfaced to ESI/atmospheric pressure chemical ionization (APCI) source for metabolomics applications.  An atmospheric pressure photoionization (APPI) source is also available for Synapt. 

Waters Q-TOF Micro
This is a quadrupole/time-of-flight mass spectrometer for MS/MS-based sequencing of peptides with a mass resolving power of ~8,000FWHM.  The Q-TOF is interfaced to a Waters CapLC HPLC system with integrated auto sampler for automated unattended nLC-MS/MS.  Determination of peptide sequences from MS/MS spectra will be through a suite of software tools including ProteinLynx Global Server (2.2), SEQUEST and MASCOT. 

Thermo Finnigan Deca XP Max Nanospray LC/MS Mass Spectrometer
This ion trap mass spectrometer is capable of extremely rapid MS/MS experiments.  Ultra-high sensitivity coupled with high resolution and fast scan speeds allows for exceptional peptide sequence coverage and metabolites quantification. This instrument is utilized for sensitive bio molecular identification and experiments requiring MSn.  It is coupled to a Michrom Paradigm MG4 multidimensional LC system with integrated auto sampler.

MICROARRAY
Scientific Advisor: Dr. Rona Scott
Genomics Research Specialist: Camille Abshire, MS, MDXT-(AAB), CLS (LSBME)

Affymetrix GeneChip System
This system is suitable for global gene expression studies using the Affymetrix GeneChip Probe arrays. Oligonucleotide arrays, prepared on glass, are hybridized to biotinylated probes prepared from biological samples and detected with a fluorescent label. Probes for these experiments are derived from a single source, and differentially expressed genes are identified by comparing the results of experiments performed with different chips. A major advantage of this approach is the ready availability of pre-prepared arrays representing a large number of sequences from a number of species.
This system consists of the following components:
1.    A GeneChip Hybridization Oven 640 for automated control of hybridization to the GeneChip arrays.
2.    A GeneChip Fluidics Station 450 for automated washing of chips and labeling of hybridized probes. This station can wash and stain four arrays simultaneously.
3.    A GeneChip Scanner 3000 7G for obtaining high-resolution images of hybridization signals. The scanner can scan 64 array simultaneously.
4.    A GeneChip Workstation that controls the operation of the system, data collection, and processing of initial raw data.
5.    A bioinformatics system, including Expression Console, Transcriptome Analysis Console, and Ingenuity Pathway Analysis.

MICROSCOPY
Scientific Advisor: Dr. Kevin Lin
Microscopy Research Specialist: Dr. Chaowei Shang

Nikon A1R Confocal & SIM Super Resolution System
This is a high-speed Nikon A1R confocal microscope combined with a SIM (Structured Illumination Microscopy) super resolution system for fixed and live sample-imaging. The Nikon Eclipse Ti-E inverted microscope is built with fully automated objectives, stage, and filter turrets. The 5 objectives range from 10X to 100X. The confocal components include 5 detectors and 4 lasers: 405nm, 488nm, 561nm, and 640nm in wavelength. The SIM components include a Hamamatsu camera and 3 LED lasers: 488nm, 561nm, and 640nm in wavelength. DAPI images can still be taken under SIM mode. The microscope is capable for bright-field imaging, multichannel fluorescence imaging, large image stitching, multipoint acquisition, time-lapse acquisition, FRAP, FRET imaging, and 2D/3D deconvolution. The resolution for conventional confocal reaches 200-250nm, while the resolution for super resolution system reaches 85-100nm. To allow long hour live cell-imaging, the microscope is housed in an incubator with CO2, O2, and humidity controls.

Leica TCS SP5 Confocal Microscope
This is a very flexible and fast confocal system for fixed or living samples. The system includes a Leica DMI 6000 CS fully automated inverted microscope with motorized stage, condenser, objective, and filter turrets. The microscope is housed in a Ludin full enclosure incubator with an internal Ludin Cube2 with CO2, O2, and humidity controls. It is equipped with 5 lasers for excitation: violet diode (405 nm), multi-line Argon (458, 476, 488, 496, and 514 nm), green HeNe (543 nm), orange HeNe (594 nm), and red HeNe (633 nm); visualization is done using a mercury arc lamp. The spectral beam splitter has freely adjustable bandwidths for the collection of signal in 5 separate detectors simultaneously or sequentially. There is also a transmitted light detector for DIC. There are 9 available objectives, ranging from a 2.5x to a Plan Apo 100x/1.46 NA oil objective. The system runs on the newest version of LAS AF software (LAS AF 2.6.3), with FRAP, FRET, Mark & Find, 3D Visualization, Colocalization, and Live Data Modes.

Zeiss AxioObserver/Apotome Microscope
The system is built around a Zeiss AxioObserver Z1 inverted fluorescent microscope, fully automated, with component recognition to minimize errors. System components include mercury arc lamp excitation, a Zeiss AxioCamMRm CCD camera with 12-bit dynamic range, extended sensitivity in the near infrared, a fully automated XYZ stage, a complement of objectives ranging from 10x to 100x, and five installed filter sets for DAPI, FITC, narrow band GFP, Rhodamine, and Far Red. The Apotome attachment is designed for precise optical sectioning. The Apotome slides easily into the optical path and projects a grid onto the image plane, which is shifted laterally in three defined steps, with an image collected at each step. A software algorithm then removes any out-of-focus signal. The acquisition software is AxioVision v.4.8, including plug-in options for Inside 4D, 3D Deconvolution, Colocalization, Mark & Find, Mosaic, and more.

Nikon Widefield Microscope
The system is built around a Nikon Eclipse TE300 inverted microscope with a range of objectives for phase, DIC, and high resolution epifluorescent imaging. The software package controls a Prior OptiscanTM XYZ stage with a full complement of stage inserts and a Prior filter wheel containing excitation filters from the Chroma 83000 filter set. This set includes single and multiband excitation filters for DAPI, FITC, GFP, Texas Red, Rhodamine, or PI. Fluorescent images are acquired with a black and white ANDOR Neo/Zyla camera; high resolution color images may also be acquired with the DS Fi2 color camera. The stage, filters, shutters, and camera are controlled by the NIS Elements software from Nikon.

Zeiss LSM 510 NLO Confocal/Multiphoton Microscope
The Zeiss LSM 510 NLO system is configured to enhance living tissue research. The scanning system is connected to an upright Axioskop 2 FS MOT microscope equipped with a set of objectives selected for physiological measurements and live animal studies. The stage remains in a fixed position, and the objectives have motorized focus control. It is equipped with the following lasers and laser lines for excitation: Argon (458, 477, 488, 514 nm), HeNe (543 nm), HeNe (633 nm), and the Coherent Chameleon-XR Ti: Sapphire laser (tunable from 705 to 980 nm). The ultrafast-pulsed Chameleon laser emitting NIR radiation allows imaging up to 500 μm deep within tissue. There are three PMTs for visible wavelength detection, a transmitted light detector, and two non-descanned detectors for multiphoton imaging. The LSM 510 ZEN operating software includes the Physiology v3.5 and  Image Visart v3.5 options that permit  2D, 3D, and 4D image collection and processing, 3D/4D animation, calibration and measurement of ion concentrations, time series analysis, and graphical mean-of-ROI analysis.
Off-line Image Analysis Stations There are two computer stations in the Core Facility Computer Lab reserved for microscope users, which are loaded with specialized imaging software. The RCFoffline2 system (Fujitsu/Siemens Celsius R650) has two processors, four terabyte hard drives, and an ATI Fire GL V7350 video card with 1 GB of on-board memory. It runs on Windows XP Professional (32 bit) and is loaded with Media Cybernetics’ AutoQuant AutoDeblur deconvolution software (AutoQuant X3), including the AutoVisualize option, and full off-line versions of Zeiss LSM 510 AIM (confocal) and Zeiss Axiovision 4.8.2 (AxioObserver with Apotome) software. RCFoffline1, a Dell Optiplex 3010, runs on Windows 7 Professional (64 bit) and is loaded with Leica LAS AF 2.6.3, NIS Elements  AR 4.13.04, and Zeiss LSM 510 ZEN 2009.

NEXT-GENERATION SEQUENCING
Scientific Advisor: Dr. Rona Scott
Genomics Research Specialist: Camille Abshire, MS, MDXT-(AAB), CLS (LSBME)

Illumina MiSeq
The Illumina MiSeq is a next-generation DNA sequencer with a single-lane flow cell. It is capable of generating up to 25 million reads with up to 15GB of output in a single run. It can produce 2 x 300 paired end reads. It has the output to accommodate targeted gene sequencing, metagenomics, small genome sequencing, targeted gene expression and amplicon sequencing. Sample libraries are loaded directly onto the MiSeq where amplified clusters are generated on the flow cell, followed by sequencing by synthesis. Samples can be barcoded and multiplexed or pooled together in the same lane.

Illumina NextSeq 550
The Illumina NextSeq 550 is a high-throughput sequencer that uses sequencing by synthesis technology, a highly accurate reversible-terminator technology.  This technology uses fluorescently labeled nucleotides to sequence hundreds of millions of clusters on a flow cell surface in parallel.  This method greatly reduces the number of errors and missed calls associated with homopolymers.  The NextSeq 550 integrates cluster generation and sequencing into a single instrument, generating base calls and quality metrics in real time.  Data are automatically transferred to the BaseSpace environment for simplified analysis, storage, and sharing.  The NextSeq 550 is flexible, offering tunable read lengths and output options to support individual research needs.  This system has a maximum output of up 120GB with up to 400 million sequencing reads.  Applications include genomic DNA sequencing, exome sequencing, mRNA sequencing, small RNA/microRNA sequencing, epigenetics, small genome sequencing and targeted resequencing.

REAL-TIME QUANTITATIVE PCR 
Scientific Advisor: Dr. Rona Scott
Genomics Research Specialist: Camille Abshire, MS, MDXT-(AAB), CLS (LSBME)

Bio-Rad CFX96
The RCF currently houses three Bio-Rad CFX96 instruments. The CFX96 is a six-channel real-time PCR system that combines advanced optical technology with precise thermal control to deliver sensitive, reliable detection. The system’s solid-state optical technology (six filtered LEDs, each with a corresponding filtered photodiode) maximizes fluorescence detection for specific dyes in specific channels, providing sensitive detection for quantification and target discrimination. Data are collected from all wells during data acquisition. At every position and with every scan, the optics shuttle is reproducibly centered above each well, so the light path is always optimal and there is no need to sacrifice data collection on one of the channels to normalize to a passive reference. Users can select multiple data acquisition modes, including a one-color fast scan for SYBR green. Thermal gradient features can be used to optimize reactions in a single run. The new CFX Manager software has advanced analysis tools for performing normalized gene expression. In addition, this system does not require fluorescein or ROX for instrument normalization.

Bio-Rad CFX384
The Research Core Facility has one Bio-Rad CFX384 Touch Real-Time PCR System.  The system's solid-state optical technology (5 filtered LEDs and 5 filtered photodiodes) provide precise quantification and multiplex target discrimination.  All other acquisition and analysis parameters are identical to the CFX96 Fast System.

RCF Policies

Using the RCF
To use any of these technologies, investigators must contact the Research Core Facility (RCF) staff to discuss issues involving use, scheduling, fees and training for each of the RCF technologies.  

Training
All users must be trained by Research Core Facility staff.  Training by other users is not sufficient.  Anyone who operates or attempts to operate RCF equipment without proper training will be subject to disciplinary action such as having their future access to the RCF technologies revoked. Training may involve anything from one or more one-on-one sessions with a staff member to formal classes developed by the RCF staff, depending on the specific technology.  Please contact the RCF staff to schedule training in the use of any of the end-user operated instruments in the Core.  Please note that training will NOT be done on a drop-in basis and MUST be scheduled ahead of time.

Data management
The ultimate responsibility for the storage of data generated from experiments or samples analyzed in the RCF belongs to the investigator.  Each investigator (or his or her staff) is provided with a printed and/or electronic copy of all analyses conducted by the staff of the RCF.  Each investigator must ensure that these data are properly stored and protected in his or her laboratory.  The RCF will make every effort to make and retain duplicate electronic copies of those data and will store them on electronic media for a period of three years.  In the event that an investigator cannot locate the original data, he or she should contact the RCF staff immediately to obtain a duplicate copy of the missing data.  In cases where data are generated by the investigator or his or her staff (on end-user operated instruments in the RCF), the individual who runs the experiment must immediately make an electronic copy of those data and keep that copy in the investigator’s lab.  Although the core facility assumes no responsibility for data files left on resident RCF computers, the staff will make every effort to back up these data files on a monthly basis.  All such files will be stored in the RCF for a period of three years.

Scientific Advisory Board

The LSU Health Shreveport Research Core Facility currently houses and maintains instrumentation for seven separate technologies. Each instrument is operated or supported by a Research Specialist and is overseen by an LSU Health Shreveport Scientific Advisor. The role of the scientific advisor is to provide investigators with assistance in the application of each technology to his or her specific experimental needs.  These advisors comprise the Scientific Advisory Board (SAB). 

Kelly Tatchell, PhD
Board Chairman
Research Core Facility Director
Associate Dean of Graduate Studies
Professor, Biochemistry & Molecular Biology 
ktatch@lsuhsc.edu 

Nick Goeders, PhD 
Faculty Representative   
Professor and Head, Pharmacology, Toxicology and Neuroscience    
Ngoede@lsuhsc.edu

Chris Kevil, PhD
Scientific Advisor, Mass Spectrometry
Vice Chancellor for Research
Dean, School of Graduate Studies
Professor, Pathology
Ckevil@lsuhsc.edu
    
Hung wen (Kevin) Lin, PhD
Scientific Advisor, Microscopy
Assistant Professor, Nuerology
Hlin2@lsuhsc.edu

Hyung Nam, PhD
Scientific Advisor, Mass Spectrometry
Assistant Professor, Pharmacology & Toxicology
hnam@lsuhsc.edu

Rona Scott, PhD
Scientific Advisor and CMTV Genomics Director
Associate Professor, Microbiology & Immunology
Rscott1@lsuhsc.edu

Matthew Woolard, PhD
Scientific Advisor, Flow Cytometry
Associate Professor, Microbiology & Immunology
mwoola@lsuhsc.edu

Research Core Facility

Research Core Facility

Research Core Facility

Research Core Facility

CONTACT US

 

Kelly Tatchell, PhD
Director, Research Core Facility
Associate Dean of Graduate Studies
ktatch@lsuhsc.edu
(318) 675-7769

 

Paula Polk, MS
Assistant Director, Research Core Facility
ppolk@lsuhsc.edu
(318) 675-4939

 

Camille Abshire, MS, MDxT-(AAB), CLS (LSBME)
Research Specialist, Genomics
cabsh2@lsuhsc.edu
(318) 675-4174

 

David Custis, MS
Research Specialist, Flow Cytometry
dcusti@lsuhsc.edu
(318) 675-4174

 

Chaowei Shang, PhD
Research Specialist, Microscopy
cshang@lsuhsc.edu
(318) 675-8537

 

Xinggui Shen, PhD
Mass Spectroscopy Specialist
Assistant Professor, Pathology
xshen@lsuhsc.edu
(318) 675-3371

 

Ana-Maria Dragoi, MD, PhD
Associate Director, INLET
Feist-Weiller Cancer Center 
adrag1@lsuhsc.edu
(318) 675-4216

 

Jennifer Carroll, PhD
Translational Research Specialist, INLET
Feist-Weiller Cancer Center 
jcarro1@lsuhsc.edu
(318) 675-4234