Arcturus XT Laser Capture Microdissection System

The Research Core Facility houses a Arcturus Laser Capture Microdissection system from Arcturus Biosciences in Room F6-13.  The Arcturus XT Laser Capture Microdissection Instrument provides an automated approach to laser microdissection of individual cells or multi-cellular structures from slides containing tissue sections. It consists of a Nikon Eclipse Ti-E inverted microscope with a solid-state near IR laser. 2x, 10x, 20x, and 40x objectives are available for use. The stage is formatted to accept up to three 1"x 3" slides.

Principles of LCM:  Laser capture microdissection (LCM) is a method used to separate individual cell types from within complex tissues.  Specifically developed caps coated with a thermoplastic film are placed on the region of interest.  The Arcturus XT instrument is then used to direct the low-powered infrared laser through the cap to melt the film onto the cells of interest.  These cells adhere to the cap when it is lifted from the tissue section – thus, capturing them for further analysis.  The laser does not affect the tissue sample; therefore, the quality of the nucleic acids and proteins within the sample are not compromised.

Molecular Devices Website:

LCM User Guide and Core Facility Policies