Sample Processing Fees:

Core Prepares Libraries
ApplicationOn Campus Off Campus
Illumina TruSeq Stranded Total RNA
Sample QC, Library Preparation and Sequencing$250$400
Data Storage (up to 1TB of data)$100$150
Data Analysis (initial analysis)*$300$400
Illumina TruSeq Stranded mRNA
Sample QC, Library Preparation and Sequencing$200$375
Data Storage (up to 1TB of data)$100$150
Data Analysis (initial analysis)*$300$400
DNA Sequencing (Illumina Nextera XT)
Sample QC, Library Preparation and Sequencing$125$250
Data Storage (up to 1TB of data)$100$150
Metagenomics/Amplicon Sequencing 
Sample QC, Library Preparation and Sequencing$50$100
Data Storage (up to 256GB of data)$40$80
*The initial analysis is $300/$400.  Any additional analysis requested is $50/hour.  The Core is only able to return lists of differentially expressed transcripts.
Submission of Prepared Libraries for Sequencing
ServiceOn CampusOff Campus
Agilent TapeStation QC and Qubit Quantification$10$20
qPCR for Library Quantification$10$20
Sample Pooling (up to 12 samples)$50$100
Normalization, Denaturing and Diluting$50$100
Sequencing Run Fee (per run)$150$300
Data Storage (up to 1TB of data)$100$150

Regardless of the application, the investigator is responsible for purchasing the Illumina sequencing reagent kit (sequencing cartridge). The price of the sequencing reagent kit depends on the read length and desired output. Please consult with Core staff prior to purchasing.

Illumina Consumable Price List - Effective April 3, 2017 (shipping costs are not reflected)
MiSeq Sequencing KitCatalog NumberCycle NumberCost
MiSeq Reagent Kit v2MS-102-200150$820
MiSeq Reagent Kit v2MS-102-2002300$1,045
MiSeq Reagent Kit v2MS-102-2003500$1,170
MiSeq Reagent Kit v3MS-102-3001150$900
MiSeq Reagent Kit v3MS-102-3003600$1,530
NextSeq 500/550 Sequencing KitCatalog Number Cycle NumberCost
NextSeq 500/550 Mid Output Kit v2FC-404-2001150$1,060
NextSeq 500/550 Mid Output Kit v2FC-404-2003300$1,700
NextSeq 500/550 High Output Kit v2FC-404-200575$1,450
NextSeq 500/550 High Output Kit v2FC-404-2002150$2,780
NextSeq 500/550 High Output Kit v2FC-404-2004300$4,450

For more information regarding Illumina sequencing kits, please visit www.illumina.com

Sample Coverage:

To determine the number of samples that can be pooled in one sequencing run, access the Illumina Sequencing Coverage Calculator through the link below. Keep in mind that the calculator uses an estimate of reads passing filter commonly found for balanced genomes (such as PhiX or the human genome). If you plan to sequence an unbalanced genome, you may have a lower number of reads passing filter, and consequently a lower output per lane.

http://support.illumina.com/downloads/sequencing_coverage_calculator.html

For more information about calculating coverage estimates, reference the technical note Estimating Sequencing Coverage.

Samples will be put in the queue for processing ONLY after they have been received and pass all quality control measures.